1.   Introduction
 
The analysis of gene expression is key to addressing a wide variety of medical  and biological research questions, including the dissection of basic biological  processes, the classification of disease, and the identification of new drug  targets. Until recently, comparing expression levels across different tissues or  cells was restricted to monitoring a few genes at […]

1. Introduction
 
 
The Invader® assay is a homogenous, isothermal, signal amplification system  for the quantitative detection of nucleic acids (1–3). The assay can directly detect  either DNA or RNA without target amplification or reverse transcription. It is  based on the ability of Cleavase® enzymes to recognize as a substrate and cleave  a specific nucleic acid structure […]

Introduction
 
With the recent completion of the human genome project, attention is now  focusing on functional genomics. In this context, a key task is to understand  normal and pathological function by empirically correlating gene expression  patterns with known and newly discovered phenotypes. As with other areas of  science, progress in this area will accelerate greatly when […]

Owing to the intense interest of many groups in determining transcript levels  in a variety of biological systems, there are a large number of methods that have  been described for gene-expression profiling. Although the actual catalog of  all techniques developed is quite extensive, there are many variations on similar  themes, and thus we have reduced […]

Introduction
 
Scientists routinely lecture and write about gene expression and the abundance  of transcripts, but in reality, they extrapolate this information from a variety  of measurements that different technologies may provide. Indeed, there are  many reasons that applying different technologies to transcript abundance may  give different results. This may result from an incomplete understanding of the […]